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File:A-Novel-Family-of-Toxoplasma-IMC-Proteins-Displays-a-Hierarchical-Organization-and-Functions-in-ppat.1001094.s007.ogv

A-Novel-Family-of-Toxoplasma-IMC-Proteins-Displays-a-Hierarchical-Organization-and-Functions-in-ppat.1001094.s007.ogv ‎(Ogg multiplexed audio/video file, Theora/Vorbis, length 49 s, 320 × 240 pixels, 110 kbps overall, file size: 653 KB)

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English: ISP1 and 3 during Toxoplasma endodyogeny. Parasites stably expressing ISP3-HA were allowed to infect HFFs and grow 24 hrs before fixation and IFA analysis. Serial sections were acquired, deconvolved and projected as a three-dimensional image. Visualization of ISP1 and ISP3 during endodyogeny shows that ISP1 is present in the maternal IMC apical cap as well as in the apical cap of each forming daughter. ISP3 exhibits different dynamics during endodyogeny: while it is clearly seen in the central and basal IMC compartments of each forming daughter, the signal has disappeared from the maternal IMC. At this stage of endodyogeny, the replicated nucleus is being segregated between the two daughter buds forming within each mother. The distinct compartments of the IMC revealed by ISP1 and 3 are already clearly visible. Red: mAb 7E8 detected by Alexa594-anti-mouse IgG. Green: anti-HA antibody detected by Alexa488-anti-rabbit IgG. Blue: Hoechst stain.
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Source Video S1 from Beck J, Rodriguez-Fernandez I, Cruz de Leon J, Huynh M, Carruthers V, Morrissette N, Bradley P (2010). "A Novel Family of Toxoplasma IMC Proteins Displays a Hierarchical Organization and Functions in Coordinating Parasite Division". PLOS Pathogens. DOI:10.1371/journal.ppat.1001094. PMID 20844581. PMC: 2936552.
Author Beck J, Rodriguez-Fernandez I, Cruz de Leon J, Huynh M, Carruthers V, Morrissette N, Bradley P
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current13:52, 17 November 201249 s, 320 × 240 (653 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 53 kbps Completed 20:46, 15 August 2018 13 s
Streaming 240p (VP9) 52 kbps Completed 04:43, 9 December 2023 1.0 s
WebM 360P 178 kbps Completed 12:32, 16 November 2023 2.0 s
Streaming 144p (MJPEG) 317 kbps Completed 08:09, 29 October 2023 2.0 s
Stereo (Opus) 872 bps Completed 18:54, 12 November 2023 1.0 s
Stereo (MP3) 48 kbps Completed 01:09, 29 October 2023 1.0 s

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