English: Detection result for YFP-Langerin in M10 stable cell lines acquired in wide field microscopy with 200ms of exposure time. The 121 stacks of size 370×404×4 voxels have been processed and are displayed using a maximum intensity projection along the z axis. This video corresponds to Fig 1a. On the right-hand side panel, for each frame, apparition events are displayed in green and vanishing events in red. The uppermost graph on the left represents the intensity inside (orange) and outside the cell (gray) as in Fig. 3. The lowermost graph represents the distribution of the minimal distance between blocks and the corresponding GEV distribution (see Fig. 4).