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File:A-Phosphatidylinositol-3-Kinase-Dependent-Signal-Transition-Regulates-ARF1-and-ARF6-during-Fcγ-pbio.0040162.sv005.ogv

A-Phosphatidylinositol-3-Kinase-Dependent-Signal-Transition-Regulates-ARF1-and-ARF6-during-Fcγ-pbio.0040162.sv005.ogv ‎(Ogg Theora video file, length 7.7 s, 360 × 120 pixels, 431 kbps, file size: 403 KB)

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English: Movie of ARF1 Activity with PI-3K Activity Blocked by LY294002 RAW264.7 macrophages co-transfected with ARF1-CFP and YFP-NGAT demonstrated high FRET signals in the area of their Golgi network in the presence of LY294002: the nucleus is visible in the phase contrast image shown at the left; next to the nucleus, the crescent-shaped region of high E D (far-right panel) corresponds to the Golgi apparatus. The Golgi is also visible in the R M image (center panel) where ARF1-CFP is present in excess over YFP-NGAT. Cells treated with LY294002 could bind opsonized particles and form phagocytic cups but could not complete phagocytosis. At the upper left and right of the macrophage, binding of opsonized erythrocytes was not accompanied by the activation of ARF1-CFP. This result indicates that amplification of FcγR-signaling by PI-3K is required for the activation of ARF1 at the phagosome.
Date
Source Video S5 from Beemiller P, Hoppe A, Swanson J (2006). "A Phosphatidylinositol-3-Kinase-Dependent Signal Transition Regulates ARF1 and ARF6 during Fcγ Receptor-Mediated Phagocytosis". PLOS Biology. DOI:10.1371/journal.pbio.0040162. PMID 16669702. PMC: 1457017.
Author Beemiller P, Hoppe A, Swanson J
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Date/TimeThumbnailDimensionsUserComment
current14:06, 16 November 20127.7 s, 360 × 120 (403 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 229 kbps Completed 01:23, 10 April 2024 4.0 s
Streaming 240p (VP9) 240 kbps Completed 05:17, 9 December 2023 2.0 s
WebM 360P 270 kbps Completed 12:43, 16 November 2023 1.0 s
Streaming 144p (MJPEG) 430 kbps Completed 08:33, 29 October 2023 1.0 s

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