Title: A contribution to the investigation of the assimilation of free atmospheric nitrogen by white and black mustard
Identifier: contributiontoin18lots (find matches)
Year: 1894 (1890s)
Authors: Lotsy, Johannes Paulus, 1867-1931; United States. Office of Experiment Stations
Subjects: Mustard; Plants Assimilation; Nitrogen
Publisher: Washington : U. S. G. P. O.
Contributing Library: U.S. Department of Agriculture, National Agricultural Library
Digitizing Sponsor: U.S. Department of Agriculture, National Agricultural Library

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15 sterilized by exposing them in glass vessels for five hours to a dry heat at a temperature of 110° C. every 3 grams being separately wrapped up in paper. After this the top of the pot was elosed by tying over it a double sheet of cotton. The pots were then placed in the dry ster- ilizer together with the following articles: (1) One glass vessel about 50 cm. high, the bottom of which was thickly covered with cotton and the opening at the top closed by a double sheet of cotton tied over it; (!') a long straight glass tube, plugged at both ends with cotton; (3) two small (J-shaped glass tubes with cotton plugged openings; (4) a glass bottle closed with a rubber stopper with two openings and cov- ered with a double sheet of cotton. All these were exposed during five hours to a temperature of 110° C. After cooling, the pot was placed
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Fig. 12.—Water cultures of. Sinapis nigra, Aug. 30, with nitrates. inside of the glass vessel on the cotton, which held it firmly in position. The cotton tied over the top of the pot was taken away and the top of the glass vessel was again closed with a double sheet of sterilized cot- ton. In this a small hole was made through which the long tube was inserted, so that its lower end rested on the sand in the pot while the upper end extended about 1 cm. outside of the vessel above the cotton. Over all a piece of parchment paper was tied with a hole in it for the tube and where this was too big it was again closed by means of a little wadding. Distilled water was put in the bottle and the two small glass tubes were put in the stopper after the manner of a wash bottle, in one end of the shorter tube a little plug of cotton remained. The tube which reached the water was now united with the tube in the glass vessel by means of a rubber tube. Finally cotton was tied around the stopper of the water bottle and also around the places where the

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