English: The movie represents one of the most entangled trajectory found in the original image of lymphocyte in a mouse lymphonodes. The choice of the connection between different sub-trajectories is only indicative of the potential application of the software. The cell is segmented on the very first image of the stack. Up to the frame 60 there is no ambiguity on the assignment of the trajectory. At this frame the original target meets a second target coming from the third quadrant of the image. At the frame 73 these two targets encounter a third target coming from the upper part of the image. From frame 80 to 190 the trajectory shows no ambiguity in the assignment. At frame 190 a second lymphocyte appears in the image and splits from the original one. This is probably due to an interaction between lymphocytes on different z planes. This trajectory is reported here only with the purpose of showing the possibility of reconstruction of complex trajectories. In the analysis of the cell interactions such a trajectory should be discarded due to the large ambiguity in the assignment of the cells. The color map of the image is an inverted gray colormap. The trajectory is reported in green levels with the same colormap as in Figs. 8, 9.