File:Advances-in-establishment-and-analysis-of-three-dimensional-tumor-spheroid-based-functional-assays-1741-7007-10-29-S6.ogv
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editDescriptionAdvances-in-establishment-and-analysis-of-three-dimensional-tumor-spheroid-based-functional-assays-1741-7007-10-29-S6.ogv |
English: Video 3. Tumor spheroid (TS)-embryoid body (EB) confrontation culture assay. 17-N-Allylamino-17-demethoxygeldanamycin (17-AAG) inhibits U-87 MG tissue invasion. Single day 4 green fluorescent protein (GFP) transduced U-87 MG TS and day 7 EBs were cocultured in each well of ultra-low attachment (ULA) 96-well plates. Cocultures were treated with 17-AAG (5 μM) or with vehicle (control) at the initiation of the cocultures. Timelapse imaging shows the invasion of U-87 MG GFP cells towards the EB in the control coculture while no invasion is evident in the 17-AAG treated culture. Images were obtained as described above over a period of 40 h. File format: MPEG. |
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Source | Vinci M, Gowan S, Boxall F, Patterson L, Zimmermann M, Court W, Lomas C, Mendiola M, Hardisson D, Eccles S (2012). "Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation". BMC Biology. DOI:10.1186/1741-7007-10-29. PMID 22439642. PMC: 3349530. | ||
Author | Vinci M, Gowan S, Boxall F, Patterson L, Zimmermann M, Court W, Lomas C, Mendiola M, Hardisson D, Eccles S | ||
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Short title | Additional file 6 |
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Author | Vinci M, Gowan S, Boxall F, Patterson L, Zimmermann M, Court W, Lomas C, Mendiola M, Hardisson D, Eccles S |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Video 3. Tumor spheroid (TS)-embryoid body (EB) confrontation culture assay. 17-N-Allylamino-17-demethoxygeldanamycin (17-AAG) inhibits U-87 MG tissue invasion. Single day 4 green fluorescent protein (GFP) transduced U-87 MG TS and day 7 EBs were cocultured in each well of ultra-low attachment (ULA) 96-well plates. Cocultures were treated with 17-AAG (5 ?M) or with vehicle (control) at the initiation of the cocultures. Timelapse imaging shows the invasion of U-87 MG GFP cells towards the EB in the control coculture while no invasion is evident in the 17-AAG treated culture. Images were obtained as described above over a period of 40 h. File format: MPEG. |
Software used | |
Date and time of digitizing | 2012 |