English: Additional file 9: Video 7 Detection of intracellular Ca2+ signals with confocal microscopy – APAP-treated primary hepatocytes displayed sustained calcium signal following ATP administration. Nuclear and cytosolic Ca2+ were monitored in individual cells by using time-lapse confocal microscopy. Primary mouse hepatocytes were cultured on glass coverslips in a density of 3×105 cells/well in 6 wells plates and kept in a Hepes-buffered solution during experiments. Cells were incubated with APAP (20 mM) and after 6 hours 4 μM cell permeant Fluo4-AM (fluo-4 acetoxymethyl ester; Molecular Probes) was added to the culture. ATP (10 μm) was used to trigger intracellular calcium signal.