English: SH-SY5Y/Neo cells were loaded with calcein as indicated above and exposed to ionomycin (2 µM). Images were acquired using a fluorescence inverted microscope (Nikon Eclipse TE-2000-S, Birlingam, CA, USA) equipped with a 150 W Xenon lamp and 100×, 1.3 numerical aperture, epifluorescence oil immersion objective and commercial software (Metamorph, Universal Imaging Corporation, Silicon Valley, CA, USA). Frames were acquired every 20 seconds over 30 minutes. Fluorescence was observed using an excitation wavelength of 485 nm and an emission wavelength of 530 nm.