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File:Cellular-Contractility-Requires-Ubiquitin-Mediated-Proteolysis-pone.0006155.s008.ogv

Cellular-Contractility-Requires-Ubiquitin-Mediated-Proteolysis-pone.0006155.s008.ogv ‎(Ogg Theora video file, length 25 s, 452 × 376 pixels, 1.23 Mbps, file size: 3.7 MB)

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English: Nocodazole induced contractions depend on proteasome activity. A. NIH3T3 cells stably expressing mCherry-α-tubulin were treated with both nocodazole and the proteasome inhibitor MG132. Nocodazole was added 4 hours before the beginning of the movie. MG132 was added at the beginning of the movie while the cell was already arrested and contracting. The cell continued to contract for an additional 7.5 hours and then ceased and remained as an arrested round mitotic cell. B. NIH3T3 cells where arrested for 15 hours with MG132 and nocodazole. They were subsequently washed and released into fresh medium without inhibitors. The cells seem to recover well and attach within about 2 hours.
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Source Movie S7 from Cinnamon Y, Feine O, Hochegger H, Bershadsky A, Brandeis M (2009). "Cellular Contractility Requires Ubiquitin Mediated Proteolysis". PLOS ONE. DOI:10.1371/journal.pone.0006155. PMID 19597551. PMC: 2705188.
Author Cinnamon Y, Feine O, Hochegger H, Bershadsky A, Brandeis M
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Date/TimeThumbnailDimensionsUserComment
current22:07, 21 November 201225 s, 452 × 376 (3.7 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 333 kbps Completed 12:54, 21 August 2018 30 s
Streaming 360p (VP9) 333 kbps Completed 01:03, 25 January 2024 1.0 s
VP9 240P 157 kbps Completed 12:54, 21 August 2018 15 s
Streaming 240p (VP9) 157 kbps Completed 20:16, 5 December 2023 1.0 s
WebM 360P 498 kbps Completed 22:08, 21 November 2012 24 s
Streaming 144p (MJPEG) 873 kbps Completed 23:40, 1 November 2023 1.0 s

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