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File:Cleaved Amplified Polymorphic Sequences (CAPS).png

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Cleaved Amplified Polymorphic Sequences (CAPS) diagram

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Description
English: Cleaved Amplified Polymorphic Sequences, or CAPS, is a molecular biology technique used to detect variations in specific regions of DNA. CAPS utilizes PCR to amplify a targeted segment of DNA, and then introduces restriction enzymes. By analyzing the banding patterns of the cut DNA fragments, scientists can identify these variations and distinguish between different genetic variants. This technique is particularly useful in applications like plant and animal breeding programs, where researchers can identify specific genes associated with desired traits.
Español: Las Secuencias Polimórficas Amplificadas y Cortadas, o CAPS por sus siglas en inglés, es una técnica de biología molecular utilizada para detectar variaciones en regiones específicas del ADN. CAPS utiliza la PCR para amplificar un segmento objetivo de ADN e introduce enzimas de restricción. Estas enzimas actúan como tijeras moleculares, cortando el ADN en secuencias de reconocimiento específicas. Si existe una variación, como un polimorfismo de un solo nucleótido (SNP), dentro del sitio de reconocimiento, la enzima podría no poder cortar o podría generar fragmentos de diferentes longitudes. Al analizar los patrones de bandas de los fragmentos de ADN cortados, los científicos pueden identificar estas variaciones y distinguir entre diferentes variantes genéticas. Esta técnica es particularmente útil en aplicaciones como programas de fitomejoramiento y cría de animales, donde los investigadores pueden identificar genes específicos asociados con los rasgos deseados.
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Author Smelgar

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