File:Cyclical-and-Patch-Like-GDNF-Distribution-along-the-Basal-Surface-of-Sertoli-Cells-in-Mouse-and-pone.0028367.s007.ogv
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Cyclical-and-Patch-Like-GDNF-Distribution-along-the-Basal-Surface-of-Sertoli-Cells-in-Mouse-and-pone.0028367.s007.ogv (Ogg Theora video file, length 32 s, 640 × 480 pixels, 656 kbps, file size: 2.51 MB)
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DescriptionCyclical-and-Patch-Like-GDNF-Distribution-along-the-Basal-Surface-of-Sertoli-Cells-in-Mouse-and-pone.0028367.s007.ogv |
English: Rotating 3D reconstruction showing the close co-localization of a GFRα1-positive spermatogonial cell with immunoreactive GDNF-positive deposits in the basal compartment of seminiferous tubule in mouse testes. PFA-fixed seminiferous tubule fragments were double-stained with anti-GDNF (green) and GFRα1 (red) antibodies (DAPI, blue) without any permeabilization steps, and then analyzed to reconstruct a three-dimensional image using an Olympus FluoView confocal laser microscope (FV10i; Olympus, Japan) in combination with Volocity software (Mitani Sangyo, Japan) (see also Fig. 7). |
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Source | Movie S2 from Sato T, Aiyama Y, Ishii-Inagaki M, Hara K, Tsunekawa N, Harikae K, Uemura-Kamata M, Shinomura M, Zhu X, Maeda S, Kuwahara-Otani S, Kudo A, Kawakami H, Kanai-Azuma M, Fujiwara M, Miyamae Y, Yoshida S, Seki M, Kurohmaru M, Kanai Y (2011). "Cyclical and Patch-Like GDNF Distribution along the Basal Surface of Sertoli Cells in Mouse and Hamster Testes". PLOS ONE. DOI:10.1371/journal.pone.0028367. PMID 22174794. PMC: 3235125. | ||
Author | Sato T, Aiyama Y, Ishii-Inagaki M, Hara K, Tsunekawa N, Harikae K, Uemura-Kamata M, Shinomura M, Zhu X, Maeda S, Kuwahara-Otani S, Kudo A, Kawakami H, Kanai-Azuma M, Fujiwara M, Miyamae Y, Yoshida S, Seki M, Kurohmaru M, Kanai Y | ||
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current | 10:03, 18 November 2012 | 32 s, 640 × 480 (2.51 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Sato T, Aiyama Y, Ishii-Inagaki M, Hara K, Tsunekawa N, Harikae K, Uemura-Kamata M, Shinomura M, Zhu X, Maeda S, Kuwahara-Otani S, Kudo A, Kawakami H, Kanai-Azuma M, Fujiwara M, Miyamae Y, Yoshida S, Seki M, Kurohmaru M, Kanai Y |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Rotating 3D reconstruction showing the close co-localization of a GFR?1-positive spermatogonial cell with immunoreactive GDNF-positive deposits in the basal compartment of seminiferous tubule in mouse testes. PFA-fixed seminiferous tubule fragments were double-stained with anti-GDNF (green) and GFR?1 (red) antibodies (DAPI, blue) without any permeabilization steps, and then analyzed to reconstruct a three-dimensional image using an Olympus FluoView confocal laser microscope (FV10i; Olympus, Japan) in combination with Volocity software (Mitani Sangyo, Japan) (see also Fig. 7). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |