English: Supplementary Movie 4 Movie of main text Fig. 3. An example EGFR entry trajectory measured in a monolayer A431 cell culture. A single labeled EGF molecule (See fluorescence bead and EGFR description) was tracked for a duration of 460 seconds. The movie playback rate is 20 times. The cell size is approximately 30 μm × 10 μm. The cell membrane is plotted as a red iso-surface while the nucleus is a blue iso-surface. In the beginning of the trajectory the molecule is membrane bound and slowly moving ~0.4 μm s-1, at approximately 150 seconds the molecule is internalized as seen by the high speed (2 μm s-1) directed motion with a total displacement of 1 μm. Trajectory is rainbow colored with blue denoting the beginning.