File:Deep-and-high-resolution-three-dimensional-tracking-of-single-particles-using-nonlinear-and-ncomms8874-s6.ogv
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DescriptionDeep-and-high-resolution-three-dimensional-tracking-of-single-particles-using-nonlinear-and-ncomms8874-s6.ogv |
English: Supplementary Movie 5 Movie of main text Fig 4. An EGFR trajectory measured in an A431 Spheroid model at a depth of 50 μm within the spheroid. A ±5 μm slice at depth 50 μm was taken with LSM, the spheroid outer plasma membrane is displayed in red iso-surface form while the nuclei are displayed as blue iso-surfaces. The total image size is 51×51 μm2. A single EGF molecule is tracked for 350 seconds, movie playback rate is 15 times. The trajectory starts on the plasma membrane and a high speed (~2 μm s-1) directed motion is observed ~250 seconds into the trajectory. The total displacement during internalization is 1 μm. Trajectory is rainbow colored with blue denoting the beginning. |
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Source | Video file from Perillo E, Liu Y, Huynh K, Liu C, Chou C, Hung M, Yeh H, Dunn A (2015). "Deep and high-resolution three-dimensional tracking of single particles using nonlinear and multiplexed illumination". Nature Communications. DOI:10.1038/ncomms8874. PMID 26219252. PMC: 4532916. | ||
Author | Perillo E, Liu Y, Huynh K, Liu C, Chou C, Hung M, Yeh H, Dunn A | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 13:19, 31 October 2016 | 45 s, 1,024 × 768 (19.12 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 5 |
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Author | Perillo E, Liu Y, Huynh K, Liu C, Chou C, Hung M, Yeh H, Dunn A |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Movie of main text Fig 4. An EGFR trajectory measured in an A431 Spheroid model at a depth of 50 μm within the spheroid. A ±5 μm slice at depth 50 μm was taken with LSM, the spheroid outer plasma membrane is displayed in red iso-surface form while the nuclei are displayed as blue iso-surfaces. The total image size is 51×51 μm2. A single EGF molecule is tracked for 350 seconds, movie playback rate is 15 times. The trajectory starts on the plasma membrane and a high speed (~2 μm s-1) directed motion is observed ~250 seconds into the trajectory. The total displacement during internalization is 1 μm. Trajectory is rainbow colored with blue denoting the beginning. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015-07-29 |