English: Direct visualisation of efficient reprogramming of astroglia into neurons by Neurog2. Time-lapse video-microscopy of an astroglia culture derived from GLAST::CreERT2/Z/EG mice following recombination in vivo between P2–P7 and transduced with a retrovirus encoding Neurog2-IRES-DsRed. The right screen reveals those cells which successfully had undergone recombination by GFP fluorescence. GFP fluorescence images were taken every 12 h after imaging had commenced (onset 18 h post-infection). The left screen shows DsRed fluorescence to reveal transduced cells. Images were taken every 6 h. Tracking of each cell was performed with bright field images taken every 4 min (not shown). DsRed- and GFP-double positive cells are marked by the red and green arrows, respectively. The beige crosses marked dead cells. High magnification views of one DsRed/GFP double-positive cell are shown in Video S2. Note the massive, partially still ongoing, reprogramming by 3 d 6 h. Time is indicated in each image.