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File:Dual-Role-of-Topoisomerase-II-in-Centromere-Resolution-and-Aurora-B-Activity-pbio.0060207.sv002.ogv

Dual-Role-of-Topoisomerase-II-in-Centromere-Resolution-and-Aurora-B-Activity-pbio.0060207.sv002.ogv ‎(Ogg Theora video file, length 14 s, 552 × 277 pixels, 277 kbps, file size: 469 KB)

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English: Time-Lapse Microscopy of S2 Cells Stably Expressing CID-GFP and RFP-H2B and Depleted for TOPO II, 72 h after the Addition of the dsRNA Z-stacks are composed of ten optical sections covering 10 μm and were acquired every 20 s. Each Z-stack is 10 μm and composed of ten optical sections. Anaphase onset corresponds to time 0 s. Merge color images of RFP-H2B (red) and CID-GFP (green) channels are shown on the left. The CID-GFP (white) channel is shown alone on the right. The two CID-GFP pairs of each centromere were identified and labeled in every layer composing the Z-stacks at the different time points. The mean distance between CID-GFP dots is approximately 1 μm. Tracking of kinetochore pairs was performed using a plug-in for the Image J software. In the video, each CID-GFP pair is labeled with the same color.
Date
Source Video S2 from Coelho P, Queiroz-Machado J, Carmo A, Moutinho-Pereira S, Maiato H, Sunkel C (2008). "Dual Role of Topoisomerase II in Centromere Resolution and Aurora B Activity". PLOS Biology. DOI:10.1371/journal.pbio.0060207. PMID 18752348. PMC: 2525683.
Author Coelho P, Queiroz-Machado J, Carmo A, Moutinho-Pereira S, Maiato H, Sunkel C
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Date/TimeThumbnailDimensionsUserComment
current23:24, 30 October 201214 s, 552 × 277 (469 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 101 kbps Completed 22:41, 24 August 2018 5.0 s
Streaming 240p (VP9) 101 kbps Completed 06:42, 17 December 2023 1.0 s
WebM 360P 135 kbps Completed 05:54, 30 November 2023 1.0 s
Streaming 144p (MJPEG) 170 kbps Completed 05:01, 9 November 2023 0.0 s

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