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File:Ectopic-A-lattice-seams-destabilize-microtubules-ncomms4094-s3.ogv

Ectopic-A-lattice-seams-destabilize-microtubules-ncomms4094-s3.ogv ‎(Ogg Theora video file, length 26 s, 320 × 132 pixels, 176 kbps, file size: 558 KB)

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English: Supplementary Movie 2 B-lattice MTs (red) were assembled using pig brain tubulin labelled with Alexa-680 and Alexa-480 fluorescent dyes and GMPCPP, and A-lattice enriched MTs (green) co-assembled with pig brain tubulin labelled with Alexa-488, Mal3FL and GMPCPP. After separate assembly reactions the B and A-lattice enriched MTs were mixed and added to a flow cell coated with rat kinesin-1 motor protein dimers. The flow cell was flushed with buffer to remove Mal3 and unbound MTs. Buffer containing ATP was added and MT motility observed by timelapse epi-fluorescence microscopy. A-lattice enriched MTs shrink faster than B-lattice MTs. Time is in minutes: seconds:milliseconds. Scale bar is 15 μm.
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Source Video file from Katsuki M, Drummond D, Cross R (2014). "Ectopic A-lattice seams destabilize microtubules". Nature Communications. DOI:10.1038/ncomms4094. PMID 24463734. PMC: 3921467.
Author Katsuki M, Drummond D, Cross R
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current00:36, 3 November 201626 s, 320 × 132 (558 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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