File:Ehrlichia-chaffeensis-Uses-Its-Surface-Protein-EtpE-to-Bind-GPI-Anchored-Protein-DNase-X-and-ppat.1003666.s010.ogv

Ehrlichia-chaffeensis-Uses-Its-Surface-Protein-EtpE-to-Bind-GPI-Anchored-Protein-DNase-X-and-ppat.1003666.s010.ogv(Ogg Theora video file, length 3.2 s, 518 × 514 pixels, 166 kbps, file size: 65 KB)

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English: DNase X is recruited to the sites of binding of rEtpE-C-coated beads on cell surface, related to Fig. 5E. Time-lapse video of rEtpE-C-coated latex beads binding to RF/6A cells ectopically expressing DNaseX-GFP. Coated beads were incubated with DNaseX-GFP-expressing RF/6A cells at 4°C, and the time 0 min was set upon raising the temperature to 37°C. The DNase X-GFP patchy signal is initially ∼3 µm away from the beads and redistributes towards the beads, eventually merging with the beads at around 5:47 min. A single z-plane, of an optical section thickness of 0.4-µm, at cell surface by deconvolution microscopy was shown. Scale bar, 30 µm.
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Source Movie S1 from Mohan Kumar D, Yamaguchi M, Miura K, Lin M, Los M, Coy J, Rikihisa Y (2013). "Ehrlichia chaffeensis Uses Its Surface Protein EtpE to Bind GPI-Anchored Protein DNase X and Trigger Entry into Mammalian Cells". PLOS Pathogens. DOI:10.1371/journal.ppat.1003666. PMC: 3789761.
Author Mohan Kumar D, Yamaguchi M, Miura K, Lin M, Los M, Coy J, Rikihisa Y
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Date/TimeThumbnailDimensionsUserComment
current15:52, 7 October 20133.2 s, 518 × 514 (65 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 53 kbps Completed 14:00, 25 August 2018 2.0 s
Streaming 480p (VP9) 54 kbps Completed 07:41, 6 February 2024 1.0 s
VP9 360P 28 kbps Completed 14:00, 25 August 2018 2.0 s
Streaming 360p (VP9) 29 kbps Completed 14:12, 7 February 2024 1.0 s
VP9 240P 17 kbps Completed 14:00, 25 August 2018 2.0 s
Streaming 240p (VP9) 18 kbps Completed 16:10, 18 December 2023 1.0 s
WebM 360P 211 kbps Completed 15:53, 7 October 2013 1.0 s
Streaming 144p (MJPEG) 101 kbps Completed 19:33, 9 November 2023 1.0 s

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