File:Electron-Tomography-of-Cryofixed-Isometrically-Contracting-Insect-Flight-Muscle-Reveals-Novel-Actin-pone.0012643.s008.ogv
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DescriptionElectron-Tomography-of-Cryofixed-Isometrically-Contracting-Insect-Flight-Muscle-Reveals-Novel-Actin-pone.0012643.s008.ogv |
English: Axial diffusion of Type 2 weak binding bridges. This movie shows Z-ward diffusion of a Type 2 weak binding myosin head as might occur during sarcomere shortening when the target zone moves M-ward. The myosin heavy chain is colored magenta to indicate a weak binding state. The myosin head starts out attached to TM in the region of actin subunit F. It then diffuses Z-ward until reaching actin subunit J at which point it is now positioned as a Type 1 weak binding myosin head and can begin the weak-to-strong transition, which largely involves azimuthal movements. When strong binding occurs, signified by change of color to red, the lever arm then moves Z-ward to complete the power stroke. Morphing done using Chimera. |
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Source | Movie S7 from Wu S, Liu J, Reedy M, Tregear R, Winkler H, Franzini-Armstrong C, Sasaki H, Lucaveche C, Goldman Y, Reedy M, Taylor K (2010). "Electron Tomography of Cryofixed, Isometrically Contracting Insect Flight Muscle Reveals Novel Actin-Myosin Interactions". PLOS ONE. DOI:10.1371/journal.pone.0012643. PMID 20844746. PMC: 2936580. | ||
Author | Wu S, Liu J, Reedy M, Tregear R, Winkler H, Franzini-Armstrong C, Sasaki H, Lucaveche C, Goldman Y, Reedy M, Taylor K | ||
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Author | Wu S, Liu J, Reedy M, Tregear R, Winkler H, Franzini-Armstrong C, Sasaki H, Lucaveche C, Goldman Y, Reedy M, Taylor K |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Axial diffusion of Type 2 weak binding bridges. This movie shows Z-ward diffusion of a Type 2 weak binding myosin head as might occur during sarcomere shortening when the target zone moves M-ward. The myosin heavy chain is colored magenta to indicate a weak binding state. The myosin head starts out attached to TM in the region of actin subunit F. It then diffuses Z-ward until reaching actin subunit J at which point it is now positioned as a Type 1 weak binding myosin head and can begin the weak-to-strong transition, which largely involves azimuthal movements. When strong binding occurs, signified by change of color to red, the lever arm then moves Z-ward to complete the power stroke. Morphing done using Chimera. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010 |