Identifier: examinationofu00saxe (find matches)
Title: Examination of the urine; a manual for students and practitioners
Year: 1909 (1900s)
Authors: Saxe, George Alexander De Santos, 1876-1911
Subjects: Urine
Publisher: Philadelphia and London, W. B. Saunders Company
Contributing Library: The Library of Congress
Digitizing Sponsor: The Library of Congress

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ative analysiswith any method the entireamount of twenty-four hoursshould be collected, meas-ured, and a sample of thethoroughly mixed urine should be examined. The amount QUANTITATIVE DETERMINATION OF SUGAR III of sugar eliminated varies a great deal according to thetime of day and the length of time after the meal. Theurine should be keptas has been describedin the introductorychapter, to preventfermentation. Quan-tities of sugar are ex-pressed usually inpercentage, but thetotal number of gramsof sugar eliminatedmust be calculated,as this is the finalcriterion. The prog-ress of the disease andthe result of treatmentmay be judged partlyby comparative studyof the quantity ofglucose eliminateddaily. The albuminshould be removedbefore making quanti-tative tests for sugar:Place 50 cc. of theurine in a porcelainevaporating dish, add2 or 3 drops of diluteacetic acid, and boilthoroughly. If albumin does not appear, add acetic acid,drop by drop, stirring constantly, and heating until the co-
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Fig. 9.—Apparatus for the quantita-tive estimation of sugar: m, Meniscus(Ogden). 112 EXAMINATION OF THE URINE agulum forms. Filter and wash the precipitate once ortwice with water. Collect the filtrate; wash with waterinto a graduate, and add enough water to make the ori-ginal 50 cc. Mix the contents of the graduate thoroughlyand use for quantitative tests. Fehlings Method.—The principle of Fehlings methodhas already been explained above (p. 100). The quantita-tive application of it consists in titrating a known quan-tity of Fehlings solution in a flask with the (diluted) urineand heating the mixture till no more copper precipitates,and until the supernatant fluid is perfectly free from bluecolor. The apparatus required is shown in Fig. 9. Theburner should be one which can be carefully regulated;the flask should have a capacity of 100 cc, and the buretshould be graduated in xV cc. The tripod should be about20 cm. high and be covered with wire gauze or asbestos. Diluting the Urine.

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