File:Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s004.ogv
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Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s004.ogv (Ogg Theora video file, length 14 s, 694 × 520 pixels, 2.35 Mbps, file size: 3.95 MB)
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DescriptionExperimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s004.ogv |
English: Time-lapse imaging of IB formation and UPS dysfunction in U87MG cells transfected with HttQ103YFP-pIRES-mRFPu. Live cell imaging was initiated at 24 hours post-transfection and images were acquired every 10 minutes. Cells were visualized under white light, and filters that detect YFP or RFP. The brightfield and fluorescence emanating from the YFP and RFP channels were merged to create a movie file. Images were acquired using a 10× objective for a total of 24 hours. At the beginning of the movie HttQ103 is expressed throughout the cell. At 36 hours (the half way point) many cells have formed an inclusion body and have notable accumulation of the red reporter protein (indicative of proteasome inhibition). Note that based on their morphology many cells with IB appear to be viable at the end of the movie. |
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Source | Video S1 from Tang M, Proctor C, Woulfe J, Gray D (2010). "Experimental and Computational Analysis of Polyglutamine-Mediated Cytotoxicity". PLOS Computational Biology. DOI:10.1371/journal.pcbi.1000944. PMID 20885783. PMC: 2944785. | ||
Author | Tang M, Proctor C, Woulfe J, Gray D | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:54, 17 November 2012 | 14 s, 694 × 520 (3.95 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Tang M, Proctor C, Woulfe J, Gray D |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010-09 |