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File:Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s006.ogv

Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s006.ogv ‎(Ogg Theora video file, length 14 s, 694 × 520 pixels, 3.11 Mbps, file size: 5.08 MB)

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English: Time-lapse imaging of U87MG cells transfected with HttQ103YFP-pIRES-mRFPu treated with PI 24 hours post-transfection showing an increase of IB formation and UPS dysfunction. Cells were visualized under white light, and filters that detect YFP or RFP. The brightfield and fluorescence emanating from the YFP and RFP channels were merged to create a movie file. Images were acquired every 10 minutes using a 10× objective for a total of 24 hours. The movie shows rapid and progressive accumulation of the mRFPu reporter protein (red colour) after the addition of PI. This coincides with the formation of IB at an earlier time point (30 hours). At the conclusion of the movie there are considerably more IB and cell death events as compared to untreated cells (videos S1 and S2).
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Source Video S3 from Tang M, Proctor C, Woulfe J, Gray D (2010). "Experimental and Computational Analysis of Polyglutamine-Mediated Cytotoxicity". PLOS Computational Biology. DOI:10.1371/journal.pcbi.1000944. PMID 20885783. PMC: 2944785.
Author Tang M, Proctor C, Woulfe J, Gray D
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This file is licensed under the Creative Commons Attribution 2.5 Generic license.
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current21:55, 17 November 201214 s, 694 × 520 (5.08 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 1.22 Mbps Completed 07:16, 26 August 2018 14 s
Streaming 480p (VP9) 1.22 Mbps Completed 02:47, 7 February 2024 1.0 s
VP9 360P 619 kbps Completed 07:16, 26 August 2018 10 s
Streaming 360p (VP9) 619 kbps Completed 23:20, 13 March 2024 1.0 s
VP9 240P 319 kbps Completed 07:16, 26 August 2018 10 s
Streaming 240p (VP9) 319 kbps Completed 03:27, 21 December 2023 1.0 s
WebM 360P 513 kbps Completed 00:52, 19 November 2012 13 s
Streaming 144p (MJPEG) 829 kbps Completed 16:29, 10 November 2023 2.0 s

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