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File:Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s011.ogv

Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s011.ogv ‎(Ogg Theora video file, length 14 s, 694 × 520 pixels, 198 kbps, file size: 340 KB)

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English: Time-lapse imaging of U87MG cells transfected with HttQ103YFP-pIRES-mRFPu pre-treated with SKF86002 two hours prior to transfection. The movie shows a decrease in IB formation and UPS dysfunction. Live cell imaging was initiated at 24 hours post-transfection and images were acquired every 10 minutes. Cells were visualized using filters that detect YFP or RFP. Fluorescence emanating from the YFP and RFP channels was merged to create a movie file. Images were acquired using a 10× objective for a total of 24 hours. Throughout the movie there are fewer cells with IBs (relative to untreated cells in videos S1 and S2). The levels of the mRFPu reporter protein (red colour) remain lower than in untreated cells and do not increase until late in the movie.
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Source Video S8 from Tang M, Proctor C, Woulfe J, Gray D (2010). "Experimental and Computational Analysis of Polyglutamine-Mediated Cytotoxicity". PLOS Computational Biology. DOI:10.1371/journal.pcbi.1000944. PMID 20885783. PMC: 2944785.
Author Tang M, Proctor C, Woulfe J, Gray D
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This file is licensed under the Creative Commons Attribution 2.5 Generic license.
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current21:56, 17 November 201214 s, 694 × 520 (340 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 89 kbps Completed 07:17, 26 August 2018 5.0 s
Streaming 480p (VP9) 89 kbps Completed 02:47, 7 February 2024 1.0 s
VP9 360P 50 kbps Completed 07:17, 26 August 2018 3.0 s
Streaming 360p (VP9) 50 kbps Completed 23:23, 13 March 2024 1.0 s
VP9 240P 30 kbps Completed 07:17, 26 August 2018 4.0 s
Streaming 240p (VP9) 30 kbps Completed 03:28, 21 December 2023 1.0 s
WebM 360P 335 kbps Completed 06:32, 18 November 2012 6.0 s
Streaming 144p (MJPEG) 185 kbps Completed 16:32, 10 November 2023 1.0 s

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