File:Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s011.ogv
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Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s011.ogv (Ogg Theora video file, length 14 s, 694 × 520 pixels, 198 kbps, file size: 340 KB)
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editDescriptionExperimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s011.ogv |
English: Time-lapse imaging of U87MG cells transfected with HttQ103YFP-pIRES-mRFPu pre-treated with SKF86002 two hours prior to transfection. The movie shows a decrease in IB formation and UPS dysfunction. Live cell imaging was initiated at 24 hours post-transfection and images were acquired every 10 minutes. Cells were visualized using filters that detect YFP or RFP. Fluorescence emanating from the YFP and RFP channels was merged to create a movie file. Images were acquired using a 10× objective for a total of 24 hours. Throughout the movie there are fewer cells with IBs (relative to untreated cells in videos S1 and S2). The levels of the mRFPu reporter protein (red colour) remain lower than in untreated cells and do not increase until late in the movie. |
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Source | Video S8 from Tang M, Proctor C, Woulfe J, Gray D (2010). "Experimental and Computational Analysis of Polyglutamine-Mediated Cytotoxicity". PLOS Computational Biology. DOI:10.1371/journal.pcbi.1000944. PMID 20885783. PMC: 2944785. | ||
Author | Tang M, Proctor C, Woulfe J, Gray D | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:56, 17 November 2012 | 14 s, 694 × 520 (340 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Tang M, Proctor C, Woulfe J, Gray D |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010-09 |