File:Fert8.JPG
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DescriptionFert8.JPG | bovine fertilization |
Date | 9/11/08 |
Source | Own work |
Author | Mtengows |
Multiple photon excitation fluorescence imaging of live bovine zona pellucida- free fertilization. Ejaculated bull sperm acrosome-reacted with PC-12 treatment and labeled with Hoechst 33342 (5 μg/mL) were exposed to zona pellucida-free oocytes and observed with transmitted light and three-photon optical sectioning fluorescence microscopy. The time course of events suggests that sperm head decondensation can be observed with Z-series collections and that cortical rearrangement occur with sperm incorporation. The exact molecules involved in these processes are not identified, but one of them may be actin. The use of this microscopy technique offers researchers the opportunity to perform observations in real time, provided appropriate excitable probes exist. Images originally published in: Tengowski, MW (2004) Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryonic development. Methods in Molecular Biology (Clifton, N.J.), 253, 165-99.
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Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled GNU Free Documentation License.http://www.gnu.org/copyleft/fdl.htmlGFDLGNU Free Documentation Licensetruetrue |
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:26, 11 September 2008 | 725 × 725 (61 KB) | Mtengows (talk | contribs) | {{Information |Description=bovine fertilization |Source=self-made |Date=9/11/08 |Author= Mtengows |Permission= |other_versions= }} Multiple photon excitation fluorescence imaging of live bovine zona pellucida- free fertilization. Ejacul |
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