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File:Fluid-Phase-Pinocytosis-of-Native-Low-Density-Lipoprotein-Promotes-Murine-M-CSF-Differentiated-pone.0058054.s014.ogv

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English: Macropinocytosis in wild-type macrophages is inhibited by PI3K and actin polymerization inhibitors. Wild-type macrophages differentiated with M-CSF were pretreated 30 min with the drugs indicated below but without serum and M-CSF. Then, macrophages were treated 30 min with fresh serum-free medium containing M-CSF (50 ng/ml) in the presence of either drug vehicle (Video S5), 100 nM wortmannin (Video S6), 4 µg/ml cytochalasin D (Video S7), or 50 µM LY294002 (Video S8). During the treatment, macrophage macropinocytosis was visualized by time-lapse, phase-contrast digital microscopy. Note that LY294002-treatment of macrophages induced small cytoplasmic vacuoles that were not formed by macropinocytosis.
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Source Videos S8 from Barthwal M, Anzinger J, Xu Q, Bohnacker T, Wymann M, Kruth H (2013). "Fluid-Phase Pinocytosis of Native Low Density Lipoprotein Promotes Murine M-CSF Differentiated Macrophage Foam Cell Formation". PLOS ONE. DOI:10.1371/journal.pone.0058054. PMID 23536783. PMC: 3594233.
Author Barthwal M, Anzinger J, Xu Q, Bohnacker T, Wymann M, Kruth H
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current21:24, 3 June 201318 s, 800 × 710 (6.96 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 903 kbps Completed 23:47, 26 August 2018 26 s
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