File:Fusion-between-Leishmania-amazonensis-and-Leishmania-major-Parasitophorous-Vacuoles-Live-Imaging-of-pntd.0000905.s002.ogv
Fusion-between-Leishmania-amazonensis-and-Leishmania-major-Parasitophorous-Vacuoles-Live-Imaging-of-pntd.0000905.s002.ogv (Ogg Theora video file, length 42 s, 320 × 240 pixels, 313 kbps, file size: 1.56 MB)
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DescriptionFusion-between-Leishmania-amazonensis-and-Leishmania-major-Parasitophorous-Vacuoles-Live-Imaging-of-pntd.0000905.s002.ogv |
English: Interaction between L. amazonensis vacuoles and L. major amastigote PVs. (A) Live imaging of macrophages previously infected with L. amazonensis-WT for 48 h and then superinfected with L. major-DsRed2 amastigotes. Video shows a macrophage, loaded with Lysotracker (green) and hosting the two species, at phase contrast and fluorescence merged channels. Image acquisition started at 72 hours after L. major-DsRed2 amastigote addition. Time after L. major-DsRed2 amastigote addition is shown (d:h:min:sec). Bar = 20 µm. (B) Live multidimensional imaging of macrophages previously infected with L. amazonensis-WT for 48 h and then superinfected with L. major-DsRed2 amastigotes. Leishmania major-DsRed2 amastigotes, sheltered by tight PV, weakly stained with Lysotracker (green), were observed at the periphery of large, Lysotracker-positive, L. amazonensis-WT PV. Multidimensional images were constructed using Imaris blend filter, and rotated in different angles. Time after L. major-DsRed2 amastigote addition is shown (d:h:min). Bar = 5 µm. |
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Source | Video S1 from Real F, Mortara R, Rabinovitch M (2010). "Fusion between Leishmania amazonensis and Leishmania major Parasitophorous Vacuoles: Live Imaging of Coinfected Macrophages". PLOS Neglected Tropical Diseases. DOI:10.1371/journal.pntd.0000905. PMID 21151877. PMC: 2998430. | ||
Author | Real F, Mortara R, Rabinovitch M | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:50, 30 October 2012 | 42 s, 320 × 240 (1.56 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Real F, Mortara R, Rabinovitch M |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Interaction between L. amazonensis vacuoles and L. major amastigote PVs. (A) Live imaging of macrophages previously infected with L. amazonensis-WT for 48 h and then superinfected with L. major-DsRed2 amastigotes. Video shows a macrophage, loaded with Lysotracker (green) and hosting the two species, at phase contrast and fluorescence merged channels. Image acquisition started at 72 hours after L. major-DsRed2 amastigote addition. Time after L. major-DsRed2 amastigote addition is shown (d:h:min:sec). Bar? |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010-12 |