File:Journal.pbio.3001783.g001.tif
Size of this JPG preview of this TIF file: 600 × 600 pixels. Other resolutions: 240 × 240 pixels | 480 × 480 pixels | 769 × 768 pixels | 1,025 × 1,024 pixels | 1,500 × 1,499 pixels.
Original file (1,500 × 1,499 pixels, file size: 558 KB, MIME type: image/tiff)
File information
Structured data
Captions
Summary edit
DescriptionJournal.pbio.3001783.g001.tif |
English: Western blotting is a standard laboratory method that uses antibodies to detect target proteins in a sample. (1) The sample, typically a mixture of proteins, is loaded on the gel. A molecular weight (MW) marker, which contains prelabeled proteins of varied, known molecular weights, is loaded on the gel alongside the protein sample as a size reference. (2) Gel electrophoresis is used to separate proteins based on their molecular weight. (3) The proteins are transferred, or “blotted”, onto a membrane. (4) The membrane is blocked to reduce nonspecific binding and then sequentially probed with a primary antibody that specifically binds to the protein of interest and a secondary antibody. The latter binds the primary antibody and carries an enzyme or a fluorophore that allows subsequent detection. (5) The signal is detected through a chemiluminescent reaction or fluorescence, respectively. (6) An image of the western blot is prepared for publication: Annotations are added and often the blot is cropped. |
Date | |
Source | Kroon C, Breuer L, Jones L, An J, Akan A, Mohamed Ali EA, et al. (2022) Blind spots on western blots: Assessment of common problems in western blot figures and methods reporting with recommendations to improve them. PLoS Biol 20(9): e3001783. https://doi.org/10.1371/journal.pbio.3001783 |
Author | Cristina Kroon, Larissa Breuer, Lydia Jones, Jeehye An, Ayça Akan, Elkhansa Ahmed Mohamed Ali, Felix Busch, Marinus Fislage, Biswajit Ghosh, Max Hellrigel-Holderbaum, Vartan Kazezian, Alina Koppold, Cesar Alberto Moreira Restrepo, Nico Riedel, Lea Scherschinski, Fernando Raúl Urrutia Gonzalez, Tracey L. Weissgerber |
Licensing edit
This file is licensed under the Creative Commons Attribution 4.0 International license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 01:43, 15 December 2022 | 1,500 × 1,499 (558 KB) | Guest2625 (talk | contribs) | Uploaded a work by Cristina Kroon, Larissa Breuer, Lydia Jones, Jeehye An, Ayça Akan, Elkhansa Ahmed Mohamed Ali, Felix Busch, Marinus Fislage, Biswajit Ghosh, Max Hellrigel-Holderbaum, Vartan Kazezian, Alina Koppold, Cesar Alberto Moreira Restrepo, Nico Riedel, Lea Scherschinski, Fernando Raúl Urrutia Gonzalez, Tracey L. Weissgerber from Kroon C, Breuer L, Jones L, An J, Akan A, Mohamed Ali EA, et al. (2022) Blind spots on western blots: Assessment of common problems in western blot figures... |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
File usage on other wikis
The following other wikis use this file:
- Usage on cs.wikipedia.org
- Usage on en.wikipedia.org
Metadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Width | 1,500 px |
---|---|
Height | 1,499 px |
Bits per component |
|
Compression scheme | LZW |
Pixel composition | RGB |
Orientation | Normal |
Number of components | 3 |
Number of rows per strip | 58 |
Horizontal resolution | 300 dpi |
Vertical resolution | 300 dpi |
Data arrangement | chunky format |
Software used | Adobe Photoshop 23.4 (Windows) |
File change date and time | 16:17, 23 September 2022 |
Color space | sRGB |