English: Z-Sections through an explanted mouse cremaster muscle, forward THG signal. Scale bar in lower left corner is 20 µm, the depth of each section is indicated in the lower right corner. The original 13 bit image (8192 gray values) was reduced to 8 bit (256 gray values) for movie generation. While visualizing less bright features, some of the brighter features became digitally overexposed during this process.