Wikimedia Commons

File:Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s013.ogv

Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s013.ogv ‎(Ogg Theora video file, length 7.7 s, 450 × 450 pixels, 433 kbps, file size: 404 KB)

Captions

Captions

Add a one-line explanation of what this file represents

Summary edit

Description
English: Example of cardiomyocytes labeled with αMHC-mCherry. The hESC line was engineered with αMHC-Puror_Rex-Neor and αMHC-mCherry_Rex-Blar vectors (Figure 1A) by G418 and Blasticidin selection (Methods). Phase contrast and fluorescence images show mCherry+ cardiomyocytes within the EB plated on gelatin-coated cell culture plastic without Puromycin treatment.
Date
Source Movie S3 from Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M (2009). "Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes". PLOS ONE. DOI:10.1371/journal.pone.0005046. PMID 19352491. PMC: 2662416.
Author Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 2.5 Generic license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
PLOS
PLOS
This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current08:08, 31 October 20127.7 s, 450 × 450 (404 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

You cannot overwrite this file.

There are no pages that use this file.

Transcode status

Update transcode status
Format Bitrate Download Status Encode time
VP9 360P 111 kbps Completed 17:29, 1 September 2018 3.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 50 kbps Completed 17:29, 1 September 2018 2.0 s
Streaming 240p (VP9) 50 kbps Completed 00:10, 15 January 2024 1.0 s
WebM 360P 457 kbps Completed 22:37, 14 November 2012 3.0 s
Streaming 144p (MJPEG) 422 kbps Completed 17:18, 9 November 2023 1.0 s

Metadata

This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s013.ogv&oldid=810817202"