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File:Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s014.ogv

Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s014.ogv ‎(Ogg Theora video file, length 5.5 s, 640 × 512 pixels, 1.49 Mbps, file size: 1,005 KB)

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English: Example of cardiomyocyte spheroid uniformly labeled with αMHC-eGFP Example of a dual drug selected CS from a hESC line engineered with αMHC-Puror_Rex-Blar and αMHC-eGFP_Rex-Neor vectors (Methods). Phase contrast and eGFP fluorescence image stacks were acquired 3 days following Puromycin drug selection (corresponding to day 16 after EB formation, see Methods).
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Source Movie S4 from Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M (2009). "Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes". PLOS ONE. DOI:10.1371/journal.pone.0005046. PMID 19352491. PMC: 2662416.
Author Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M
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current08:08, 31 October 20125.5 s, 640 × 512 (1,005 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 483 kbps Completed 17:30, 1 September 2018 7.0 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 217 kbps Completed 17:29, 1 September 2018 5.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 90 kbps Completed 17:29, 1 September 2018 3.0 s
Streaming 240p (VP9) 91 kbps Completed 00:11, 15 January 2024 11 s
WebM 360P 498 kbps Completed 01:28, 19 November 2012 7.0 s
Streaming 144p (MJPEG) 864 kbps Completed 17:18, 9 November 2023 1.0 s

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