File:Ligand Probe Crosslinker Immobilization.png
Size of this preview: 588 × 599 pixels. Other resolutions: 236 × 240 pixels | 471 × 480 pixels | 754 × 768 pixels | 1,005 × 1,024 pixels | 2,010 × 2,048 pixels | 3,000 × 3,057 pixels.
Original file (3,000 × 3,057 pixels, file size: 629 KB, MIME type: image/png)
Captions
Captions
Illustration of target identification using various immobilization strategies.
Summary edit
| Description |
English: A. A small molecule is derivatized for immobilization on a solid support, such as a magnetic bead or affinity column. Protein targets bind to the small molecule and can be identified after elution.
B. A small molecule is attached to an enrichment handle. The enrichment handle is then used to pull down the small molecule-protein complex. C. A small molecule molecule is functionalized with a cross-linking group and an enrichment handle. After a target binds the small molecule, the cross-linking group can be activated, often by light, forming a covalent bond between target and small molecule. The enrichment handle is then used to pull down the small molecule-protein complex. |
| Date | |
| Source | BioRender.com |
| Author | BioRender.com |
Licensing edit
This file is licensed under the Creative Commons Attribution-Share Alike 4.0 International license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.
File history
Click on a date/time to view the file as it appeared at that time.
| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 18:23, 6 December 2021 | | 3,000 × 3,057 (629 KB) | Sgold626 (talk | contribs) | Uploaded a work by BioRender.com from BioRender.com with UploadWizard |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
