File:Lipopolysaccharide-O-Antigen-Prevents-Phagocytosis-of-Vibrio-anguillarum-by-Rainbow-Trout-pone.0037678.s006.ogv
Lipopolysaccharide-O-Antigen-Prevents-Phagocytosis-of-Vibrio-anguillarum-by-Rainbow-Trout-pone.0037678.s006.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 16 s, 640 × 480 pixels, 918 kbps overall, file size: 1.76 MB)
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DescriptionLipopolysaccharide-O-Antigen-Prevents-Phagocytosis-of-Vibrio-anguillarum-by-Rainbow-Trout-pone.0037678.s006.ogv |
English: Live-cell microscopy video demonstrating that the V. anguillarum Δwzt mutant carrying the wild-type wzt gene regains the ability to evade phagocytosis by the rainbow trout skin epithelial cells. Skin epithelial cells were isolated from rainbow trout and infected with 105 bacteria ml−1. Following infection, phagocytic activities of motile epithelial cells were tracked using live-cell microscopy. This video is representative of epithelial cell interactions also associated with the Δwzm and ΔwbhA mutants carrying the respective complementing wild-type gene. All three complemented mutant strains regained the ability to evade phagocytosis. |
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Source | Video S6 from Lindell K, Fahlgren A, Hjerde E, Willassen N, Fällman M, Milton D (2012). "Lipopolysaccharide O-Antigen Prevents Phagocytosis of Vibrio anguillarum by Rainbow Trout (Oncorhynchus mykiss) Skin Epithelial Cells". PLOS ONE. DOI:10.1371/journal.pone.0037678. PMID 22662189. PMC: 3360773. | ||
Author | Lindell K, Fahlgren A, Hjerde E, Willassen N, Fällman M, Milton D | ||
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current | 11:40, 15 November 2012 | 16 s, 640 × 480 (1.76 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Live-cell microscopy video demonstrating that the V. anguillarum ?wzt mutant carrying the wild-type wzt gene regains the ability to evade phagocytosis by the rainbow trout skin epithelial cells. Skin epithelial cells were isolated from rainbow trout and infected with 105 bacteria ml?1. Following infection, phagocytic activities of motile epithelial cells were tracked using live-cell microscopy. This video is representative of epithelial cell interactions also associated with the ?wzm and ?wbhA mutants carrying the respective complementing wild-type gene. All three complemented mutant strains regained the ability to evade phagocytosis. |
Software used | |
Date and time of digitizing | 2012 |