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File:Live-cell-imaging-techniques-to-study-T-cell-trafficking-across-the-blood-brain-barrier-in-vitro-2045-8118-10-7-S3.ogv

Live-cell-imaging-techniques-to-study-T-cell-trafficking-across-the-blood-brain-barrier-in-vitro-2045-8118-10-7-S3.ogv ‎(Ogg Theora video file, length 11 s, 553 × 416 pixels, 1.43 Mbps, file size: 1.84 MB)

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English: Movie 3. Shear-resistant arrest, polarization, crawling and diapedesis of CD4+ T cells on and across TNFα stimulated wt pMBMECs under flow (high magnification). Movie corresponds to the evaluation shown in Figure 2b. The experimental setup was identical to that described in Movie 1. Flow increase to physiological shear stress (1.5 dyne/cm2) was at 8 min (lower timer). Numbers placed on T cells visible on one frame of the movie (lower timer = 40 sec) were assigned for identification of each individual T cell. Images were taken with a 20x objective (Objective LD “Plan-Neofluar” 20x/0,4 Korr Ph2 M27) under phase-contrast illumination at 3 images per min; recording time 21 min; movie taken at 6 images per sec; field of view 438 μm x 329 μm.
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Source Coisne C, Lyck R, Engelhardt B (2013). "Live cell imaging techniques to study T cell trafficking across the blood-brain barrier in vitro and in vivo". Fluids and Barriers of the CNS. DOI:10.1186/2045-8118-10-7. PMID 23336847. PMC: 3560242.
Author Coisne C, Lyck R, Engelhardt B
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Date/TimeThumbnailDimensionsUserComment
current21:31, 9 February 201311 s, 553 × 416 (1.84 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 357 kbps Completed 00:44, 2 September 2018 4.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 190 kbps Completed 00:44, 2 September 2018 4.0 s
Streaming 240p (VP9) 190 kbps Completed 23:13, 16 January 2024 1.0 s
WebM 360P 510 kbps Completed 21:35, 9 February 2013 8.0 s
Streaming 144p (MJPEG) 433 kbps Completed 00:37, 10 November 2023 1.0 s

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