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File:Live-cell-imaging-techniques-to-study-T-cell-trafficking-across-the-blood-brain-barrier-in-vitro-2045-8118-10-7-S4.ogv

Live-cell-imaging-techniques-to-study-T-cell-trafficking-across-the-blood-brain-barrier-in-vitro-2045-8118-10-7-S4.ogv ‎(Ogg Theora video file, length 21 s, 352 × 288 pixels, 567 kbps, file size: 1.42 MB)

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English: Movie 4. Initial contact of CD8+ T cells with the spinal cord microvasculature during EAE. At the beginning, the inflamed spinal cord microvasculature is visualized by TRITC-Dextran in the circulation of a C57/BL6 mouse affected with EAE within one field of view (FOV) at x10 objective. The spinal cord white matter microvasculature is visualized above the large spinal cord collecting vein, always seen at the bottom of each video frame. After switching the fluorescence filter on the microscope, the infusion of 1 aliquot (1.3 x 106 cells/100μL) of CellTracker green CD8+ T cells, pretreated for 20 min with rat IgG isotype control, is started and T cells can be observed either passing through the corresponding vascular beds or interacting as rolling or capturing to the microvasculature in real time (objective x10) within one FOV.
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Source Coisne C, Lyck R, Engelhardt B (2013). "Live cell imaging techniques to study T cell trafficking across the blood-brain barrier in vitro and in vivo". Fluids and Barriers of the CNS. DOI:10.1186/2045-8118-10-7. PMID 23336847. PMC: 3560242.
Author Coisne C, Lyck R, Engelhardt B
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current21:31, 9 February 201321 s, 352 × 288 (1.42 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 108 kbps Completed 00:44, 2 September 2018 8.0 s
Streaming 240p (VP9) 108 kbps Completed 23:13, 16 January 2024 1.0 s
WebM 360P 220 kbps Completed 21:10, 1 December 2023 2.0 s
Streaming 144p (MJPEG) 355 kbps Completed 00:37, 10 November 2023 1.0 s

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