File:Microfluidic-Chips-for-In-Vivo-Imaging-of-Cellular-Responses-to-Neural-Injury-in-Drosophila-Larvae-pone.0029869.s006.ogv
Microfluidic-Chips-for-In-Vivo-Imaging-of-Cellular-Responses-to-Neural-Injury-in-Drosophila-Larvae-pone.0029869.s006.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 11 s, 787 × 576 pixels, 1.14 Mbps overall, file size: 1.52 MB)
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DescriptionMicrofluidic-Chips-for-In-Vivo-Imaging-of-Cellular-Responses-to-Neural-Injury-in-Drosophila-Larvae-pone.0029869.s006.ogv |
English: Laser microsurgery in the SI-chip of sensory neuron dendrites labeled with G-CaMP3.0. UAS-G-CaMP3.0 was expressed in class IV neurons using the ppk-Gal4 driver. Single neurons were imaged by spinning disc confocal microscopy at 5 frames/second. While imaging, a pulsed UV laser was used to transect a primary dendritic branch. The movies were false colored and the scale bar indicates the relative intensity level of G-CaMP3.0. Laser transection induced a rapid increase in G-CaMP intensity, which began at the site of injury and travelled to the cell body. |
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Source | Movies S1 from Ghannad-Rezaie M, Wang X, Mishra B, Collins C, Chronis N. "Microfluidic Chips for In Vivo Imaging of Cellular Responses to Neural Injury in Drosophila Larvae". PLOS ONE. DOI:10.1371/journal.pone.0029869. PMID 22291895. PMC: 3264548. | ||
Author | Ghannad-Rezaie M, Wang X, Mishra B, Collins C, Chronis N | ||
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current | 07:02, 19 October 2012 | 11 s, 787 × 576 (1.52 MB) | Open Access Media Importer Bot (talk | contribs) | Uploaded with the Open Access Media Importer. (test edit) botrequest |
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Author | Ghannad-Rezaie M, Wang X, Mishra B, Collins C, Chronis N |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Laser microsurgery in the SI-chip of sensory neuron dendrites labeled with G-CaMP3.0. UAS-G-CaMP3.0 was expressed in class IV neurons using the ppk-Gal4 driver. Single neurons were imaged by spinning disc confocal microscopy at 5 frames/second. While imaging, a pulsed UV laser was used to transect a primary dendritic branch. The movies were false colored and the scale bar indicates the relative intensity level of G-CaMP3.0. Laser transection induced a rapid increase in G-CaMP intensity, which began at the site of injury and travelled to the cell body. |
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Date and time of digitizing | 2012-01-23 |