File:Molecular-mechanisms-of-NET-formation-and-degradation-revealed-by-intravital-imaging-in-the-liver-ncomms7673-s4.ogv
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DescriptionMolecular-mechanisms-of-NET-formation-and-degradation-revealed-by-intravital-imaging-in-the-liver-ncomms7673-s4.ogv |
English: Supplementary Movie 3 Staining for extracellular DNA (extDNA) and its removal by DNase. Intravascular DNA was labeled with Sytox green (shown by green fluorescencebright) and neutrophils with AF750-anti-Ly6G antibody (magenta). At the indicated time point, 2000 U DNase was infused i.v., and the digestion of extDNA could be observed by the loss of intravascular Sytox Greenbright staining. The scale bar indicates 20 μm. The video was recorded for approximately 13 minutes, for details see the time stamp. |
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Source | Video file from Kolaczkowska E, Jenne C, Surewaard B, Thanabalasuriar A, Lee W, Sanz M, Mowen K, Opdenakker G, Kubes P (2015). "Molecular mechanisms of NET formation and degradation revealed by intravital imaging in the liver vasculature". Nature Communications. DOI:10.1038/ncomms7673. PMID 25809117. PMC: 4389265. | ||
Author | Kolaczkowska E, Jenne C, Surewaard B, Thanabalasuriar A, Lee W, Sanz M, Mowen K, Opdenakker G, Kubes P | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 14:53, 1 November 2016 | 17 s, 1,280 × 720 (4.25 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 3 |
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Author | Kolaczkowska E, Jenne C, Surewaard B, Thanabalasuriar A, Lee W, Sanz M, Mowen K, Opdenakker G, Kubes P |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Staining for extracellular DNA (extDNA) and its removal by DNase. Intravascular DNA was labeled with Sytox green (shown by green fluorescencebright) and neutrophils with AF750-anti-Ly6G antibody (magenta). At the indicated time point, 2000 U DNase was infused i.v., and the digestion of extDNA could be observed by the loss of intravascular Sytox Greenbright staining. The scale bar indicates 20 μm. The video was recorded for approximately 13 minutes, for details see the time stamp. |
Software used | |
Date and time of digitizing | 2015-03-26 |
Language | English |