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File:Molecular-mechanisms-of-NET-formation-and-degradation-revealed-by-intravital-imaging-in-the-liver-ncomms7673-s5.ogv

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English: Supplementary Movie 4 DNase is efficiently removing extDNA but not neutrophil elastase (NE) or histones (H2A.X). ExtDNA is shown in bright green (Sytox Green), histone H2A.X in red (AF555-anti-H2A.X antibody), and neutrophil elastase in blue (AF647-anti-NE antibody). All channels (green-blue-red) were recorded at the same time but are shown here separately for clarity. Part I: removal of extDNA, part II: the effect of DNase on NE, part III: the impact of DNase on H2A.X. The scale bars indicate 20 μm. The video was recorded for approximately 10 minutes, for details see the time stamp.
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Source Video file from Kolaczkowska E, Jenne C, Surewaard B, Thanabalasuriar A, Lee W, Sanz M, Mowen K, Opdenakker G, Kubes P (2015). "Molecular mechanisms of NET formation and degradation revealed by intravital imaging in the liver vasculature". Nature Communications. DOI:10.1038/ncomms7673. PMID 25809117. PMC: 4389265.
Author Kolaczkowska E, Jenne C, Surewaard B, Thanabalasuriar A, Lee W, Sanz M, Mowen K, Opdenakker G, Kubes P
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current14:53, 1 November 201638 s, 1,280 × 720 (6.16 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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