English: Single-Cell Observations of Persistence Following Heat Induction for the hipA7 λcI857KnR Lysogens Superposition of phase-contrast and fluorescence images acquired by time-lapse microscopy of the hipA7 strain carrying the λcI857KnR prophage during and after heat induction. Fluorescence expression of the pR′-tR′-gfp is monitored. At t = 0, lysogenic bacteria are exposed to 42 °C for 80 min. The temperature is then set back to 32 °C. Videos were acquired in parallel at several different locations. At the end of the measurement, no survivors are observed in the wt (Video S1), whereas a number of dividing cells can be seen in the hipA7 strain. The times between frames are indicated in Text S2. Images were flattened and filtered using High Gauss (ImagePro).