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File:Novel-Approach-for-the-Detection-of-the-Vestiges-of-Testicular-mRNA-Splicing-Errors-in-Mature-pone.0057296.s006.ogv

Novel-Approach-for-the-Detection-of-the-Vestiges-of-Testicular-mRNA-Splicing-Errors-in-Mature-pone.0057296.s006.ogv ‎(Ogg multiplexed audio/video file, Theora/Vorbis, length 12 s, 320 × 240 pixels, 1.51 Mbps overall, file size: 2.17 MB)

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English: Observation of spermatozoa after Percoll purification. The frozen-thawed ejaculated spermatozoa (from bull #2) were washed once in a two-step gradient of 2 mL of 60% and 5 mL of 30% isotonic Percoll that was prepared with PBS, and subsequently washed once in PBS-PVA by centrifugation. After centrifugation, the sperm pellet was suspended with PBS-PVA and observed on a heated stage at 38.5°C under a bright-field microscope.
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Source Movie S1 from Noda T, Sakase M, Fukushima M, Harayama H (2013). "Novel Approach for the Detection of the Vestiges of Testicular mRNA Splicing Errors in Mature Spermatozoa of Japanese Black Bulls". PLOS ONE. DOI:10.1371/journal.pone.0057296. PMID 23468960. PMC: 3582612.
Author Noda T, Sakase M, Fukushima M, Harayama H
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current20:37, 6 September 201312 s, 320 × 240 (2.17 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 391 kbps Completed 04:33, 5 September 2018 14 s
Streaming 240p (VP9) 308 kbps Completed 05:56, 6 February 2024 1.0 s
WebM 360P 941 kbps Completed 02:49, 2 December 2023 3.0 s
Streaming 144p (MJPEG) 1.1 Mbps Completed 07:08, 16 November 2023 1.0 s
Stereo (Opus) 82 kbps Completed 07:09, 16 November 2023 1.0 s
Stereo (MP3) 126 kbps Completed 07:09, 16 November 2023 1.0 s

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