English: Supplementary Movie Individual blastomere isolation method. This movie is a demonstration of how individual blastomeres were isolated from embryos for STRT sequencing. The movie shows a representative day 3 embryo (8-cell stage) kept in place with a holding pipette. Laser pulses were applied to degrade zone pellucida; the diameter of the target area is approximately 40 μm. The movie subsequently shows change of optics for a wider-angle image and aspiration of individual blastomeres from the embryo. All eight blastomeres were recovered intact and placed in the culture medium. Not shown in the movie is the transfer of single cells into plate wells containing STRT lysis buffer.