File:Phosphatidylinositol-(45)-Bisphosphate-Controls-T-Cell-Activation-by-Regulating-T-Cell-Rigidity-and-pone.0027227.s008.ogv
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Phosphatidylinositol-(45)-Bisphosphate-Controls-T-Cell-Activation-by-Regulating-T-Cell-Rigidity-and-pone.0027227.s008.ogv (Ogg Theora video file, length 20 s, 76 × 75 pixels, 20 kbps, file size: 50 KB)
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editDescriptionPhosphatidylinositol-(45)-Bisphosphate-Controls-T-Cell-Activation-by-Regulating-T-Cell-Rigidity-and-pone.0027227.s008.ogv |
English: A representative interaction of sensor-transduced 5C.C7 T cell with a CH27 B cell lymphoma APC in the presence of 10 µM MCC agonist peptide is shown in this and all subsequent movies. Movies were acquired using time-lapse epifluorescence microscopy. Differential interference contrast (DIC) images are shown on top, with matching top-down, maximum projections of 3-dimensional sensor fluorescence data on the bottom. The sensor fluorescence intensity is displayed in a rainbow-like false-color scale (increasing from blue to red). 20 s intervals in movie acquisition are played back as 2 frames per second. The individual captions list for each movie the figure it refers to, the sensor and any non-fluorescent additional proteins expressed by the 5C.C7 T cell, the time of T cell/APC couple formation given both as the movie frame number and the displayed movie time (in parentheses), and noteworthy features of the movie. Movie S1 refers to Fig. S1C , the 5C.C7 T cells are transduced with PIP5K α-GFP, cell coupling occurs in frame 3 (1s), and prominent are a large lamellum upon cell coupling and transient interface accumulation of PIP5K α-GFP. |
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Source | Movie S1 from Sun Y, Dandekar R, Mao Y, Yin H, Wülfing C (2011). "Phosphatidylinositol (4,5) Bisphosphate Controls T Cell Activation by Regulating T Cell Rigidity and Organization". PLOS ONE. DOI:10.1371/journal.pone.0027227. PMID 22096541. PMC: 3214035. | ||
Author | Sun Y, Dandekar R, Mao Y, Yin H, Wülfing C | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 02:31, 26 April 2017 | 20 s, 76 × 75 (50 KB) | Revent (talk | contribs) | attempt to fix transcodes | |
06:19, 21 October 2012 | 20 s, 76 × 75 (44 KB) | Open Access Media Importer Bot (talk | contribs) | Uploaded with the Open Access Media Importer. (test edit) botrequest |
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Image title | Acquired from PI Exposure: 200 ms Region: 1300 x 1030, offset at (0, 0) Binning: 2 x 2 Bit Depth: 12-bit (5 MHz) Speed: Fast 8-Bit Copy of Copy of dic large |
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Language | eng |
Date and time of digitizing | 14:14, 31 March 2010 |