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File:Plexin-B2-Negatively-Regulates-Macrophage-Motility-Rac-and-Cdc42-Activation-pone.0024795.s006.ogv

Plexin-B2-Negatively-Regulates-Macrophage-Motility-Rac-and-Cdc42-Activation-pone.0024795.s006.ogv ‎(Ogg Theora video file, length 6.2 s, 800 × 600 pixels, 1.16 Mbps, file size: 875 KB)

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English: Live cell video of cell motility of Plxnb2−/−, M-CSF treated macrophages. Representative cell movement videos over a 2.5 hour time period. 1×104 cells were plated on glass bottom dishes (MatTek Ashland, MA P35G-1.5-10-C) in complete DMEM, rested overnight, and treated with 50 ng/ml M-CSF in warmed DMEM. Cells were imaged on a Nikon Biostation (Belmont, CA) with a 20× objective at ten different points. Images were collected every 5 minutes for a total time of 2.5 hours following sham treatment. Three separate movies were filmed and 45 cells scored for velocity. Cell velocity was analyzed using manual tracking in ImageJ [76]. The experiment was repeated three separate times using cells from different mice in each experiment.
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Source Video S4 from Roney K, O'Connor B, Wen H, Holl E, Guthrie E, Davis B, Jones S, Jha S, Sharek L, Garcia-Mata R, Bear J, Ting J (2011). "Plexin-B2 Negatively Regulates Macrophage Motility, Rac, and Cdc42 Activation". PLOS ONE. DOI:10.1371/journal.pone.0024795. PMID 21966369. PMC: 3179467.
Author Roney K, O'Connor B, Wen H, Holl E, Guthrie E, Davis B, Jones S, Jha S, Sharek L, Garcia-Mata R, Bear J, Ting J
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current03:30, 14 November 20126.2 s, 800 × 600 (875 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 390 kbps Completed 22:17, 13 October 2018 4.0 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 243 kbps Completed 22:17, 13 October 2018 3.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 147 kbps Completed 22:17, 13 October 2018 2.0 s
Streaming 240p (VP9) 147 kbps Completed 06:52, 6 December 2023 1.0 s
WebM 360P 427 kbps Completed 07:32, 15 November 2012 4.0 s
Streaming 144p (MJPEG) 202 kbps Completed 04:35, 19 November 2023 1.0 s

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