English: Inhibition of Gαi exchange decreased 2XFYVE-GFP accumulation. HeLa cells were transfected with 2X-FYVE GFP and mCherry-tubulin and treated with PTX (200 ng/ml for 3 h prior to the experiment, right panel) or its vehicle (left panel) and imaged on a confocal microscope (5 s acquisition for a 22 slices z-stack, 1 image every 3 min). 3D reconstruction was performed using Imaris and movies (2 frames per second) were synchronized using Adobe Premiere. Scale bar is 5 µm.