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File:Surface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv012.ogv

Surface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv012.ogv ‎(Ogg Theora video file, length 21 s, 320 × 256 pixels, 2.34 Mbps, file size: 5.89 MB)

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English: Cytochalasin D Treatment Does Not Affect Calcium Flux Stimulated by Antibody Crosslinking AD10 T cells treated with 10 μM cytochalasin D for 1 h at 37 °C were attached on poly-l-lysine–coated cover glasses in a flow chamber; 10 μg/ml biotinylated anti-Vβ3 monoclonal antibody, KJ25, was introduced and incubated for 10 min. A few T cells fluxed calcium at low levels during incubation. The antibody was then washed away, and 10 μg/ml streptavidin was introduced. Crosslinking by streptavidin led to high-level calcium flux in most of the cells (the last quarter of the video). Calcium levels are displayed in pseudocolor. The speed of the video is 50 times real time.
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Source Video S12 from Ma Z, Sharp K, Janmey P, Finkel T (2008). "Surface-Anchored Monomeric Agonist pMHCs Alone Trigger TCR with High Sensitivity". PLOS Biology. DOI:10.1371/journal.pbio.0060043. PMID 18303949. PMC: 2253636.
Author Ma Z, Sharp K, Janmey P, Finkel T
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This file is licensed under the Creative Commons Attribution 2.5 Generic license.
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current14:50, 16 November 201221 s, 320 × 256 (5.89 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 321 kbps Completed 00:52, 21 October 2018 17 s
Streaming 240p (VP9) 321 kbps Completed 03:34, 17 December 2023 1.0 s
WebM 360P 520 kbps Completed 13:49, 2 December 2023 2.0 s
Streaming 144p (MJPEG) 824 kbps Completed 20:35, 19 November 2023 1.0 s

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