File:Surface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv013.ogv

Surface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv013.ogv(Ogg Theora video file, length 24 s, 320 × 256 pixels, 882 kbps, file size: 2.48 MB)

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English: Cytochalasin D Treatment Does Not Affect Calcium Flux Stimulated by Antibody Crosslinking: Results of Cytochalasin D Introduced Together with Streptavidin Untreated AD10 T cells were attached on poly-l-lysine–coated cover glasses in a flow chamber. The untreated AD10 T cells were incubated with KJ25 antibody first, then 10 μg/ml streptavidin was introduced together with 10 μM cytochalasin D. A few T cells fluxed calcium at low levels during incubation. Crosslinking by streptavidin led to high-level calcium flux in most of the cells (the last quarter of the video). Calcium levels are displayed in pseudocolor. The speed of the video is 50 times real time.
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Source Video S13 from Ma Z, Sharp K, Janmey P, Finkel T (2008). "Surface-Anchored Monomeric Agonist pMHCs Alone Trigger TCR with High Sensitivity". PLOS Biology. DOI:10.1371/journal.pbio.0060043. PMID 18303949. PMC: 2253636.
Author Ma Z, Sharp K, Janmey P, Finkel T
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Date/TimeThumbnailDimensionsUserComment
current14:50, 16 November 201224 s, 320 × 256 (2.48 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 277 kbps Completed 00:52, 21 October 2018 15 s
Streaming 240p (VP9) 277 kbps Completed 03:34, 17 December 2023 0.0 s
WebM 360P 396 kbps Completed 13:49, 2 December 2023 2.0 s
Streaming 144p (MJPEG) 571 kbps Completed 20:35, 19 November 2023 2.0 s

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