English: Migration with Less Cell Contact. Occasionally, the cell sheet migrates without maintaining significant cell-cell contact, as it does in this movie. We presume that this is due to unstable flow of trypsin, which then briefly compromised cell-cell contact. This can occur if the inlets had become partially occluded with cells. Alternatively this can happen if during the trypsin treatment, somewhere upstream from where we are imaging, cells are cleaved from the surface in large groups, also altering the flow profile, causing downstream digestion of cell-cell contacts. Cells are stained with CMFDA.