File:The-C-Proteins-of-Human-Parainfluenza-Virus-Type-1-Block-IFN-Signaling-by-Binding-and-Retaining-pone.0028382.s014.ogv
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The-C-Proteins-of-Human-Parainfluenza-Virus-Type-1-Block-IFN-Signaling-by-Binding-and-Retaining-pone.0028382.s014.ogv (Ogg Theora video file, length 27 s, 640 × 480 pixels, 805 kbps, file size: 2.56 MB)
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DescriptionThe-C-Proteins-of-Human-Parainfluenza-Virus-Type-1-Block-IFN-Signaling-by-Binding-and-Retaining-pone.0028382.s014.ogv |
English: Supplemental video file for Figure 8 (M6RP and Stat1). Vero cells were infected with F170S HPIV1 for 48 h, mock-stimulated with medium containing neither FCS nor IFN-β for 1 h and subsequently fixed, permeabilized and stained for nucleus (DAPI, blue), M6PR (green) and Stat1 (red). Images of cross-sections (z-stacks) of 0.17 µm thickness were acquired on a Leica SP5 confocal microscope and 3D reconstructions were generated using Imaris software (Bitplane, Zurich, Switzerland). The first of the two rotations of the 3D reconstruction shows all three channels as indicated above, the second rotation shows the blue channel (DAPI, nucleus) and the “co-localization channel” (yellow, calculated from the co-localizing signals of the red and the green channels). |
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Source | Video S11 from Schomacker H, Hebner R, Boonyaratanakornkit J, Surman S, Amaro-Carambot E, Collins P, Schmidt A (2012). "The C Proteins of Human Parainfluenza Virus Type 1 Block IFN Signaling by Binding and Retaining Stat1 in Perinuclear Aggregates at the Late Endosome". PLOS ONE. DOI:10.1371/journal.pone.0028382. PMID 22355301. PMC: 3280236. | ||
Author | Schomacker H, Hebner R, Boonyaratanakornkit J, Surman S, Amaro-Carambot E, Collins P, Schmidt A | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 17:36, 14 November 2012 | 27 s, 640 × 480 (2.56 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Schomacker H, Hebner R, Boonyaratanakornkit J, Surman S, Amaro-Carambot E, Collins P, Schmidt A |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |