File:The-Herpes-Virus-Fc-Receptor-gE-gI-Mediates-Antibody-Bipolar-Bridging-to-Clear-Viral-Antigens-from-ppat.1003961.s005.ogv
The-Herpes-Virus-Fc-Receptor-gE-gI-Mediates-Antibody-Bipolar-Bridging-to-Clear-Viral-Antigens-from-ppat.1003961.s005.ogv (Ogg Theora video file, length 2.9 s, 512 × 512 pixels, 2.46 Mbps, file size: 871 KB)
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DescriptionThe-Herpes-Virus-Fc-Receptor-gE-gI-Mediates-Antibody-Bipolar-Bridging-to-Clear-Viral-Antigens-from-ppat.1003961.s005.ogv |
English: 4-D movie of ABB-dependent trafficking of gD and anti-gDhFc to lysosomes (corresponds to Figure 3A). Live cell imaging of HeLa cells expressing gE-gI and gD-Dendra2 (green) incubated with EGF (red) and anti-gDhFc (blue). Regions of EGF-gD colocalization appear yellow; regions of gD-IgG colocalization appear cyan, regions of EGF-IgG colocalization appear magenta, and regions of triple colocalization appear white. 4-D multi-channel confocal imaging was performed using a 63× oil objective lens (αPlan-APOCHROMAT 1.45 Oil DIC) on a LSM510 microscope (Zeiss) and an electron-multiplying charge-coupled device (CCD) camera (Hamamatsu Photonics), controlled by the ZEN 2009 software (Zeiss). Z-stacks (at 1 µm section thickness and up to 16 µm total depth) were captured approximately every 3 min for ∼90 min. The video was recorded at a time resolution of approximately 5 seconds per frame and presented at 10 frames per second. The equatorial planes for z-stack sections are shown on this video. |
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Source | Movie S1 from Ndjamen B, Farley A, Lee T, Fraser S, Bjorkman P (2014). "The Herpes Virus Fc Receptor gE-gI Mediates Antibody Bipolar Bridging to Clear Viral Antigens from the Cell Surface". PLOS Pathogens. DOI:10.1371/journal.ppat.1003961. PMID 24604090. PMC: 3946383. | ||
Author | Ndjamen B, Farley A, Lee T, Fraser S, Bjorkman P | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 07:10, 15 March 2014 | 2.9 s, 512 × 512 (871 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Ndjamen B, Farley A, Lee T, Fraser S, Bjorkman P |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014-03 |