English: Growth of GAL-MPS3 in YPGR. HDEL-dsRed marks the nuclear membrane and ER while Pus1-GFP highlights the nucleus in cells containing GAL-MPS3 grown in YEP plus 2% raffinose and 2% galactose at 23°C. From ∼75–90 min, the upper left cell undergoes mitosis, where the nucleus is observed to form an hour-glass shape and then elongate between the mother and daughter cell. Following the completion of mitosis, the nucleus becomes round again. Another mitosis was observed in the cell in the bottom right between ∼130–160 min. At later time points, a few changes in nuclear morphology were observed but overall, the nuclei largely retained a relatively normal shape throughout the 3.5 h time course. Bar, 5 µm.