English: Microinjection of recombinant protein VI-M1 displaces Daxx from PML bodies to the cytoplasm. U2OS cells were cotransfected with Daxx-mCherry (left panel) and PML-GFP (middle panel) expression plasmids (superimposed signal on the right) and cultivated at 37°C on a heated stage in CO2 stabilized medium attached to a SP5 confocal microscope equipped with a microinjection device (Eppendorf). The left cell of two cotransfected cells with similar expression levels of both proteins was then microinjected into the cytoplasm using recombinant bacterially expressed and purified protein VI-M1 at a final concentration of 0.3 µg/µl. Prior to injection, cells were imaged using the SP5 confocal microscope at 20 s intervals for 10 frames at a single optical section with pinhole setting of two using a 20× magnification and maximum resolution. Injection was performed manually under optical control in DIC mode and immediately after images were taken at 20 s intervals for further 40 frames without changing the optical setting to minimize the time between injection and imaging (time delay ∼1–2 min). Please note the increase of PML intranuclear dynamics in the injected cell that starts as soon as the Daxx signal diminishes. This dynamic was seen in all cells injected with VI-wt or with VI-M1 but never in buffer injected control cells.