File:Transportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s010.ogv

Transportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s010.ogv(Ogg Theora video file, length 13 s, 512 × 512 pixels, 2 Mbps, file size: 3.01 MB)

Captions

Captions

Add a one-line explanation of what this file represents

Summary edit

Description
English: HMM-propelled actin filaments coated with streptavidin attached to the filaments via biotinylated actin. Actin filaments (5 nM; monomer concentration; 4 biotins/actin) were immobilized on HMM in the flow cell and then incubated with 20 nM TRITC-labelled streptavidin for 1 min. The concentration of free biotin to block unoccupied biotin-sites of streptavidin was 40 µM. The actin filaments were also labelled with Alexa-488 phalloidin. The movie first illustrates the TRITC-labelled streptavidin using a TRITC filter set followed by observation of the entire filaments using a FITC filter set at the end. Main Fig. 1B extracted from movie. Frame rate: 5 s−1 (real time) and image size: 85×85 µm2.
Date
Source Movie S2 from Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A (2013). "Transportation of Nanoscale Cargoes by Myosin Propelled Actin Filaments". PLOS ONE. DOI:10.1371/journal.pone.0055931. PMID 23437074.
Author Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 2.5 Generic license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
PLOS
PLOS
This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current14:40, 4 March 201313 s, 512 × 512 (3.01 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

There are no pages that use this file.

Transcode status

Update transcode status
Format Bitrate Download Status Encode time
VP9 480P 619 kbps Completed 02:29, 24 October 2018 7.0 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 289 kbps Completed 02:29, 24 October 2018 6.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 118 kbps Completed 02:29, 24 October 2018 4.0 s
Streaming 240p (VP9) 118 kbps Completed 00:01, 21 December 2023 1.0 s
WebM 360P 510 kbps Completed 14:41, 4 March 2013 9.0 s
Streaming 144p (MJPEG) 322 kbps Completed 13:28, 10 November 2023 1.0 s

Metadata