File:Transportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s010.ogv
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Transportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s010.ogv (Ogg Theora video file, length 13 s, 512 × 512 pixels, 2 Mbps, file size: 3.01 MB)
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DescriptionTransportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s010.ogv |
English: HMM-propelled actin filaments coated with streptavidin attached to the filaments via biotinylated actin. Actin filaments (5 nM; monomer concentration; 4 biotins/actin) were immobilized on HMM in the flow cell and then incubated with 20 nM TRITC-labelled streptavidin for 1 min. The concentration of free biotin to block unoccupied biotin-sites of streptavidin was 40 µM. The actin filaments were also labelled with Alexa-488 phalloidin. The movie first illustrates the TRITC-labelled streptavidin using a TRITC filter set followed by observation of the entire filaments using a FITC filter set at the end. Main Fig. 1B extracted from movie. Frame rate: 5 s−1 (real time) and image size: 85×85 µm2. |
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Source | Movie S2 from Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A (2013). "Transportation of Nanoscale Cargoes by Myosin Propelled Actin Filaments". PLOS ONE. DOI:10.1371/journal.pone.0055931. PMID 23437074. | ||
Author | Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:40, 4 March 2013 | 13 s, 512 × 512 (3.01 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |